Mccoy’s media 5A Medium with 50 ml FB Essence, 5 ml glutaminG, 5 ml strep(1x),Phosphate-Buffered saline solution (PBS), Trypsin, 25 ml flask, 12 millimolars creatine, eppendorf centrifuge,pipet, incubator 27°C 80% CO2, Class 2 type A/B3 30% exhaust & 70% recirculating air Nuaire hood, 70% Ethanol spray bottle, paper towels,cell counter,10 microliter cell solution, 10 microliter trypsin, 1 ml pipet tips, vortex gene touch mixer, tube rack,conical, 3 T25 flasks, markers, conical, beaker, Medium, 12 well plate, mini eppendorf tubes, trypan blue, cell plate.