An estimated amount of one million people in the united states alone receive chemotherapy every year. The lifetime probability of being diagnosed with cancer is 39.7% for men and 37.6% for women, which is a little more than 1 in 3. With an average of 4700 cases of cancer being diagnosed every day, chemotherapy is the most used treatment (source 8). Tumors are neoplasms or an abnormal buildup of tissue mass, which can turn into cancer and spread through the body. Chemotherapy is one of the most effective ways of killing cancer cells, but it comes at a great price: endless side effects. Chemotherapy is usually injected directly into the vein or orally, it works by targeting cells that divide quickly and killing them, consequently it kills healthy cells in the process (source 3). The death of the healthy fast-growing cells such as bone marrow, skin, intestines and hair cells is what leads to the adverse side effects of chemotherapy (source 7). The main effects of chemotherapy are bone loss, nerve damage, weakening of the heart, blood disorders, weakness in hands and feet, digestive distress and brain fog (source 4). The detrimental effects of chemotherapy can be seen in MAT B III, an immortal breast cancer cell line in rats, which can be used to model breast cancer ( source 1). Doxorubicin is a chemotherapy drug currently being used to kill the MAT B III cells in rats. It works by disrupting the topoisomerase-2-mediated DNA repair which is why it is used in treatments against solid tumors and hematological malignancies (source 6). Doxorubicin comes with the several side effects such as a progressive decline in muscular function and increased muscular fatigue and heart failure ( source 6). Although there is a way to mitigate these side effects and this is with Creatine Monohydrate. Creatine is the product of a two-step reaction which is synthesized in the liver and kidneys (source 2). Creatine helps build muscle by regenerating adenosine triphosphate( ATP) which increases fuel leading to an increase in muscle size, strength, and power (source 5). Therefore using Creatine after the use of Doxorubicin can help mitigate the side effects and help regain muscle strength in patients. Although there is limited research examining the effects of creatine supplementation on tumor dynamics the purpose of the current study is to examine the effects of creatine incubation on MAT B3 growth rates. Creatine monohydrate should have no effect on the growth of MAT B3 cells because Creatine is not a metabolic pathway for the MAT B3 cells to grow since creatine only indirectly affects the tissue distribution of enzymes (source 5).