On observation the wells with 2 millimolar of creatine monohydrate grew more MAT B3 cells than the control group which was not hypnotized, this most likely occurred because of human error. For example, one possible way the data could be inaccurate is because of over trypsinization which leads to cell lysis. Another reason could have been the cells were cryopreserved incorrectly as they are shared with several other people in the lab. Additionally, the cells may have been thawed incorrectly, which is stressful for cells. All these factors and more could have introduced more variants in the data.
There was no data from C1 Creatine added day 7, C2 Creatine added day 7, C3 control day 7 and C4 control day seven because the wells grew mold in the incubator as shown in figure 2. The mold growth could have been caused by several different factors such as unsterile techniques or the incubator being contaminated. For the A4 cell after putting the conical in the centrifuge and spinning the old media was not poured out before putting the cells in 10 microliters of trypan blue making the data inaccurate. For wells, A3 and B1 trypsin were added to well B1 from A3 contaminating both the wells so data could not be derived from either of those wells. As shown in well A1 and well A2 they were both wells grown for 1 day with creatine monohydrate supplementation yet there is a 44.4% percent change between the two as A1 grew 54,000 MAT B3 cells and A2 grew 78,000 MAT B3 cells. This shows how inconstant the data from the wells are. Although the data didn’t match up with my hypothesis there was a large room for error as mentioned earlier and there would need to be several more trials to show that creatine supplementation causes an increase in MAT B3 cell growth. |
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